Introduction

RTPrimerDB is a public database for primer and probe sequences used in real-time PCR assays employing popular chemistries (SYBR Green I, Taqman, Hybridisation Probes, Molecular Beacon) to prevent time-consuming primer design and experimental optimisation, and to introduce a certain level of uniformity and standardisation among different laboratories.

We strongly encourage researchers to submit their validated primer and probe sequence, so that other users can benefit from their expertise. The database can be queried using the official gene name or symbol, Entrez or Ensembl Gene identifier, SNP identifier, or oligonucleotide sequence.

Different options make it possible to restrict a query to a particular application (Gene Expression Quantification/Detection, DNA Copy Number Quantification/Detection, SNP Detection, Mutation Analysis, Fusion Gene Quantification/Detection, Chromatin immunoprecipitation (ChIP)), organism (Human, Mouse, Rat, and others) or detection chemistry. Data submission is allowed after free registration whereby you obtain a login name and password.

Currently, 8761 real-time PCR assays for 6017 genes are available, submitted by 240 people.

Last submission: link

Publications
  • PATTYN, F., SPELEMAN, F., DE PAEPE A. & VANDESOMPELE, J. (2003). RTPrimerDB: the Real-Time PCR primer and probe database. Nucleic Acids Research, 31(1): 122-123. [Download]
  • PATTYN, F., ROBBRECHT, P., SPELEMAN, F., DE PAEPE A. & VANDESOMPELE, J. (2006). RTPrimerDB: the Real-Time PCR primer and probe database, major update 2006. Nucleic Acids Research, 34(Database issue): D684-688. [Download]
  • LEFEVER S, VANDESOMPELE J, SPELEMAN F, PATTYN F. (2008). RTPrimerDB: the portal for real-time PCR primers and probes. Nucleic Acids Research, Oct 23. [Epub ahead of print] [Download]